Virulence characterization of isolated fungal antagonist against Rice blast (Magnaporthe oryzae) Couch [manuscript]
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Date
2019
Authors
Jeffrey M. Orejana
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Abstract
Rice blast caused by Magnaporthe oryzae is one of the most important fungal disease in rice. Bio-control agent provides sustainable control for the disease. Generally, the study aimed to characterize the virulence of isolated fungal antagonist against rice blast. Moreover, in this study the rice blast fungal pathogen (Magnaporthe oryzae) was isolated through single spore isolation technique. Identified the fungal antagonist using morphological and molecular approach, and conducted dual culture assay.
Results showed that P1, P2, P6 and P8 isolates were identified as Penicillium verruculosum and siolates FA and FB were identified as Fusarium oxysporum with 98-100% identity. Phylogenetic tree displayed that the P8J (P8) isolates was closely related to P. verruculosum species among the four Penicillium species and FAJ was closely related to Fusarium oxysporum. Dual culture assay showed that among five isolates, P1, P2, P6, FA and FB the P1 isolates exhibited the highest percent growth of inhibition of 46.9% against Magnaporthe oryzae compare to the other isolates.