Major practice in micropropagation of Medinilla spp. at Benguet State University, la Trinidad, Benguet [manuscript]
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Date
2017
Authors
Katrina Joyce M. Lacson
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Abstract
The major practice in microprogation od Medinilla spp. was conducted at the Regional Tissue Culture Laboratory Benguet State University (BSU), Barangay Cabanao, Balili, la Trinidad Benguet from December 21, 2016 to January 21, 2017. The objective of the major practice (MP) was enable the MP students to acquire firsthand experience in tissue culture technologies on ornamental particularly in Medinilla. the specific objectives were, to know the specific procedures in tissue culture and gain knowledge on both experiential and technical areas, to conduct mini experiments that will enable the major practice students to: a) develop expertise in inoculation procedures b) acquire more precise ability and technical knowledge in preparing stock solutions c) gain hands on experience in mericloning or subcloning of Medinilla d) become familiar with in ex vitro acclimatization of plantlets grown in in vitro-
During the major practice, the student was exposed to inoculation, mericloning and acclimatization of Medinilla. Activities undertaken were preparation of stock solution, preparation of cultures media, cooking of cultured media, sterilization of cultured media, sterilization of contamination, collection of explants, sterilization of explants, inoculation, mericloning and acclimatization.