Diversity of Rhizoctonia solani causing rice sheath blight in selected rice based farming system in Nueva Ecija

dc.contributor.authorRizajoy C. Bulatao
dc.date.accessioned2025-06-10T06:25:15Z
dc.date.available2025-06-10T06:25:15Z
dc.date.issued2018
dc.description.abstractA total of 30 isolates of Rhizoctonia solani AG1-1A were collected from different rice based farming systems in Nueva Ecija. After subjecting to molecular identification, results showed that 8 isolates were classified as Rhizoctonia oryzae and 22 isolates were Rhizoctonia solani AG1-1A. R. solani isolani were tested for diversity using VNTR analysis. Bands were assessed using NTSYSpc 2.0. Six isolates were found identical with 0.88 similarity coefficient from cluster I and II. However, remaining subgroup isolates from both cluster I and II were diverse having a range of 0.65 to 0.85 similarity coefficient. Twenty two isolates of R. solani AG1-1A were subjected to pathogenicity test by determining relative lesion height, disease incidence (%) and diseases severity (%). Seven (7) days after inoculation (DAI), seven isolates, SJCPAL 1 RC SJCPAL 1 RC, SCMM1 RR, SCMM2 RR, SCMBAN 1 RR, SCMBAN2 RR and SCMBAN4 RR did not able to produce lesions while the other 15 isolates produced necrotic lesion. Fourteen (140 days after inoculation, all isolates were able to caused typical symptom of sheath blight with irregular shaped dark brown lesions. Results also showed that all the isolates were aggressive producing more than 60% disease severity at 21 days after inoculation and among the 22 isolates, only 3 of them, SJCC 1 RC, SCMBS 1 RR and SCMBS 2 RR, resulted to 100% infection, where necrosis across the entire leaf and sheath section was observed.
dc.identifier.urihttp://granarium.clsu.edu.ph/handle/123456789/204
dc.language.isoen_US
dc.titleDiversity of Rhizoctonia solani causing rice sheath blight in selected rice based farming system in Nueva Ecija
dc.typeThesis
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