Optimum nutritional conditions for the efficient mycelial growth and antioxidant activity of Collybia reinakeana P. Henn Urdaneta strain on broth media from natural sources [manuscript]
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Date
2018
Authors
Joshua C. Ramos
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Abstract
Collybia reinakeana P. Henn is a wild endemic mushroom in the philippines. Cultures of C. reinakeana collected from Urdaneta, Pangasinan were used and the optimal liquid media for mycelial biomass production and antioxidant activity was investigated. Among six culture media types, both rice bran and oat meal produced the most luxuriant mycelia, 15 and 30 days after incubation, whereas mungbean recorded the least mycelial biomass. Antioxidant activity of C. reinakeana Urdaneta strain was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay by means of Trolox equivalent antioxidant capacity (TEAC). The highest radical scavenging activity was found in mungbean (15 days) with antioxidant activity of 3.6mg TE/g, whose values is higher than the control Trolox (2.84mg TE/g). Rice bran (30 days) recorded the least antioxidant activity with 0.91 mg TE/g.