Isolation and molecular identification of freshwater microalgae in Paitan Lake, Cuyapo, Nueva Ecija
| dc.contributor.author | Maria Angelika D. Colangan | |
| dc.date.accessioned | 2025-10-16T01:16:12Z | |
| dc.date.available | 2025-10-16T01:16:12Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Microalgae is a diverse group of unicellular prokaryotic and eukaryotic microorganisms that comonly thrives in marine and freshwater environment. It is a potential source of food supplement and bio-materials used in pharmaceutical industry. This study focused on the isolation and molecular identification of microalgae present in Paitan Lake, Cuyapo Nueva Ecija. Isolation of microalgae was carried out though dilution method. Regular microscopic observation was undergone to monitor the occurrence of unialgal cell per container. The isolated microalgae was then morphologically characterized to determine its preliminary identity based from phenotypic features. To increase the sensitivity of the identification and to confirm the identity of the isolates, genomic DNA extraction was followed up. The template DNA extracted was amplified using 16s rDNA primer. The PCR product was sent to first base laboratory in Malaysia for sequencing. Phylogenetic tree was also concerned to uphold and verify the similarity of the sequenced isolates with the related species in the GenBank data base. A total of four (4) species of microalgae were successfully isolated and morphologically characterized. these are Leptolynhya sp. (ASI), Scenedesmus sp (AO), Monoraphiduim sp. (AS2), and Chlorella sp. (AB). Three out of four isolates were successfully amplified using 16s gene marker. ASI was successfully sequenced while the remaining samples showed negative result in the sequencing procedure. It was confirmed in the BLAST analysis that the sample had 83.54% similarity to Leptolyngbya sp. which coincides with its initial morphological identification. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/755 | |
| dc.language.iso | en | |
| dc.relation.supervisor | EDEN S. DAVID, Ph.D. | |
| dc.title | Isolation and molecular identification of freshwater microalgae in Paitan Lake, Cuyapo, Nueva Ecija | |
| dc.type | Thesis |