Neem leaves (Azadiracha indica A. Juss) crude extract against guava fruit fly (Bactrocera dorsalis Hendel) [manuscript]
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Date
2020
Authors
Jocelyn T. Neuda
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Abstract
Use of synthetic pesticides to control fruit flies in farming could be hazardous to human health and damage to the environment. An alternative to these synthetic agents are natural products that can be derived from various plant sources such as the neem tree leaves. Thus, this study was conducted to determine the total phenolic content of the leaves of neem (Azadirachta indica A. Juss) and test the adulticidal and larvicidal activity of the crude extract against guava fruit fly (Bactrocera dorsalis Hendel). Leaves of Azadirachta indica were extracted with 95% ethanol. Thin layer chromatography screening was conducted to identify the phytochemicals in the crude extract. Total phenolic content (TPC) was measured using the Folin-Ceiocalteu method. Phenolic compound such as flavonoids, tannins and phenols, triterpenes ans anthraquinones are found present in the neem leaf crude extract. The total phenolic content of Azadirachta indica leaf crude extract was 152.47 mg gallic acid equivalents (GAE)/g. Table 5 shows the percent mortality of Trial 3. Lowest percent mortality was recorded in the control after 24 hours with 53.3% in adulticidal assay. The treatments (1.25, 2.5, 5.0, 7.5 and 10%) neem crude extrat seems to be more effective than the control after 24 hours. Emergence of living fruit flies also affected by the concentration of neem. Higher concentrations (10%, 7.5% and 5%) has no emergence of living fruit flies. The bioactivity can be attributed to the phytochemical components identified. Azadirachta indica exhibit adulticidal and larvicidal property against guava fruit flies and can be used as alternative plant-based pesticides.