Identification and distribution of antimicrobial resistance in E. coli and Salmonella sp. from meat (Pork, Beef, and Chicken) in Western Visayas (Region VI [manuscript]
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Date
2019
Authors
Mara Regina D. Uyehara
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Abstract
The study was conducted to identify and characterize antimicrobial-resistant Escherichia coli and Salminelle sp. isolated from meat samples in Western Visayas (Region VI). Specifically, those isolated from pork, chicken and beef samples were supermarkets and wet markets in the region. A total of thirty (30) meat samples were collected from Aklan of Region 6 (Western Visayas). Fifteen (15) samples came from wet markets and fifteen (15) samples came from supermarkets. E. coli were initially cultivated in BHI broth and transferred to MacConkey agar. Colonies from E. coli plates were transferred in differential and selective media (TSI medium, LIM medium, MR-VP medium and Simmons citrate). Salmonella sp. were initially cultivated in BPW broth and Rappaport-Vassidialis broth then transferred to XLD agar. Colonies from Salmonella sp. plates were transferred in differential and selective media (TSI medium and LIM medium). Identified colonies E. coli and Salmonella sp. were then subjected to antimicrobial susceptibility testing. Results reveal that majority of the E. coli and Salminella sp. colonies isolated from meat (pork, chicken and beef) samples showed patterns of resistance to antibiotics.