Identification and evaluation of antagonistic fungal epiphytes against the rice blast pathogen [manuscript]
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Date
2017
Authors
Donn Luigi S. Castro
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Abstract
This study aimed to determine the antagonistic fungal epiphytes as potential biological control agents against the rice blast pathogen (magnaporthe oryzae B. Couch) through In vitro culture. The fungal epiphytes were also characterized morphologically for identification of genera.
There were 66 fungal epiphytes isolated from different rice varieties collected in different municipalities of Nueva Ecija: Cabanatuan City, Palayan City, general Tinio, PeƱaranda, Guimba, Cuyapo, Talugtug and Lupao with rice varieties NSIC RC 132H, NSIC RC 132H, NSIC Rc-222, NSIC-Rc 218, NSIC-Rc 152, NSIC Rc 222, PSB-Rc 130 snd GSR-12 respectively. Eleven (11) epiphytic fungal isolates were slected as potential biological control agents that inhibit the growth of M. oryzae.
The top most promising fungal epiphytes were Mucor sp. CaT4 with percent inhibition of 67.36%, followed by Mucor sp. LB2 (62.69%), Penicillium sp. CaT (37.33%), Aspergillus sp. LM1 (33.68%), Fusarium sp. CuB1 (33.15%), Curvularia sp. GT4 (32.46%), Curvularia sp. CaM7 (31.91%), Fusarium sp. CuM2 (28.91%), Curvularia sp. LM2 (28.49%), Curvularia sp. LT2 (28.08%) and Fusarium sp. GtM2 (26.29%) with the least inhibitory activity.
All epiphytic fungal isolates were properly characterized morphologically (colony and micro structure). These where then identified all the genus level.