Quality assessment of postmortem epididymal sperm of non-descript cattle (Bos taurus) before and after dilution in semen extender [manuscript]
| dc.date.accessioned | 2026-04-27T06:59:14Z | |
| dc.date.available | 2026-04-27T06:59:14Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | The study recovered spididymal sperm from non-descript cattle and determined the duration of its viability prior to and after the addition of semen extender stored at refrigerated temperature (4 to 5°C) conditions. Epididymal sperm was collected from three non-descript cattle at a local abbatoir in Baler, Aurora. After the animals had been properly slaughtered, epididymides were then examined, sliced and cleaned for swim-up. The collected epididymal sperm wass checked for volume, comcentration, motility and morphology 24 hours after the addition of extender. Results showed that the immediately recovered epididymal sperm can be viable for up to 24 hours post-mortem after the addition of extender. Moreover, the sperm volume of 0.41ml and sperm concentration of 99x10⁷ were still considered acceptable given the amount of time that had lapsed after the animal was slaughtered and the transport duration from the slaughterhouse to the laboratory. Sperm motility was reduced from 26.67±6.79 to 12.50±3.82 after 24 hours post-mortem at refrigerated temperature. There was also a noticeable decrease in the number of live sperm with the subsequent rise in the dead sperm cell numbers even with the addition of semen extender and storage at refrigerated temperature. Cytoplasmic drolets, pyriform head, detached head, bent tail and coiled tail were found in the epididymal sperm samples which may or may not greatly affect the fertility of the bull. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1169 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | MARVIN BRYAN S. SALINAS, DVM | |
| dc.title | Quality assessment of postmortem epididymal sperm of non-descript cattle (Bos taurus) before and after dilution in semen extender [manuscript] | |
| dc.type | Thesis |