Identification and evaluation of enzymatic ability of fungal endophytes in Citrofortunella microcarpa (Bunge) Wijnands
| dc.contributor.author | Rommel Dave C. Reyes | |
| dc.date.accessioned | 2025-06-11T02:59:06Z | |
| dc.date.available | 2025-06-11T02:59:06Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Citrofortunella microcarpa, belonging to family Rutaceae, is one of the most important commercial fruit crops grown in the Philippines. Endophytes are found in various plant tissue types. Endophytic fungi produce valuable enzymes that are relatively unexplored which are useful to pharmaceutical and agricultural industries. Thus, this study was conducted to evaluate the enzymatic ability of endophytic fungi isolated from C. microcarpa stem and leaves that would provide an information on the diversity and ability to produce fungal enzymes. The present study evaluated the fungal enzymatic ability of fungal endophytes found in C. microcarpa leaves and stem. Fungal isolates were identified based on their cultural and morphological caharacteristics. then, their identity were confirmed using molecular methods. isolated fungi were tested for their ability to produce amylase, cellulase, laccase and protease. A total of 11 fungal endophytes where isolated from stem and leaves of Citrofortunella microcarpa namely: Collectrichum fracticola, Collectotrichum siamense, Fusarium oxysporium, lasiodiplodia theobromae, Nigrospora oryzae, Nigrospora rubi, Nodulisporium indicum, Colletorichum, gloeosporiodes, Phomposis azadirachtae, Phyllosticta capitalensis, and an unidentified species under the Order Pleosporales. All the endophytic fungal isolates showed production of amylases. The highest production of amylase were from P. capitalensis, followed by the unidentified species and Nigrospora oryzae. Furthermore, a total of four species of endophytic fungi namely: L. theobromae, N. oryzae, C. gloeosporioides and the unidentified species had potential for cellulose degradation. The species of fungi that had the largest enzymatic index for cellulose activity was the unidentified species of Pleosporales. meanwhile, among the tested organisms for the extracellular protease activity positive results were observed in the unidentified species. F. oxysporium and P. azadirachtae were producer of laccase enzyme. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/243 | |
| dc.language.iso | en_US | |
| dc.subject | Research Subject Categories::NATURAL SCIENCES::Biology | |
| dc.subject | Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Microbiology | |
| dc.title | Identification and evaluation of enzymatic ability of fungal endophytes in Citrofortunella microcarpa (Bunge) Wijnands | |
| dc.type | Thesis |