Production of protein hydrolysate from by-products of milkfish (Chanos chanos) processing [manuscript]
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Date
2020
Authors
Cyril Joy V. Balais
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Abstract
Annually, there are approximately tons of by-products produced resulting from substantial harvesting of marine resources. Different strategies were devised to convert these by-products in a beneficial and nutritive product such as the hydrolysis of fish protein. In this study, production of fish protein hydrolysate (EPH) through acid hydrolysis was carried out using mixed-by products consisting of viscera and bones from the processing of milkfish (Chanos chanos). Acid hydrolysis using 4M, 6M and 8M HCI was conducted for 90 minutes under high pressure (15 psi) at 120 degree celcius before the samples were oven-dried for 48 h at 80 degrees celcius. the different functional properties of FPH were investigated. The results showed that treating milkfish bones and viscera with 4M, 6M and 8M HCI can yield 5.141%, 6.083% and 5.510% of FPH, respectively. Although the concentration of acid did not affect the amount of FPH produced, it was found to influence the degree of hydrolysis (DH). The FPH from 8M HCI treatment had the highest DH (43.877%) while the one produced by 4M HCI has the lowest DH (22.048%). All FPHs were not as effective as ascorbic acis as antioxidant, since their EC50 values (741.2-1448 mg protein/ml) were several orders of magnitude higher than the value for the positive control (1.939 mg/ml). In general, the FPH samples obtained from 6M and 8M HCI treatments were more soluble across different pH compared to the FPH from 4M HCI. The maximum solubility of the different FPH prepared using 4M, 6M, and 8M HCI were at 70.211%+10.098, 82.439%+4.533% and 94.910+7.191, respectively. The FPH from 8M HCI treatment was found to have the highest emulsifying stability index (43.831 min at pH 6) and emulsifying activity index (53.509m2/g at pH 8) while the FPH from 6M HCI had the highest foaming capacity (223.333% at pH 8) and foaming stability (208.333% at pH 10). Taken together, the results suggest that even though similar amounts of FPH can be obtained using 4M, 6M and 8M HCI, the FPHs from 6M and 8M HCI treatments have better functional properties.