Optimization of liquid culture conditions of Pleurotus cystidiosus as source of mycochemicals and antioxidants [manuscript]
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Date
2018
Authors
Hzasell S. Abalos
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Abstract
Pleurotus cystidiosus has a unique biology and one of the most interesting of all the oyster mushrooms because of its convenience and accessibility. However, it remains uncultivated for mycelial biomass production. Thus, the present work established the optimum liquid culture condition for the mycelial biomass production of P. cystidiosus for elucidation of functional activities. In order to provide information on the biomass production and biological activities of P. cystidiosus, optimization of liquid culture conditions, analysis of radical scavenging activity and total phenolics and mycochemical screening were analyzed. The coconut water with pH 6.5 incubated in room temperature (30°C), alternating light and dark, and static condition were the ideal cultural conditions for the mycelial growth of P. cystidiosus. Mycelia produced in agitated condition registered the highest radical scavenging activity of 35.55%, while the culture spent recorded 31.22%. However, mycelia produced in static condition exhibited 31.39% scavenging activity while its spent had 17.80%. Agitated spent recorded higher total phenolic content (112.13 mg GAE/g sample), while the mycelia produced gave 34.00 mg GAE/g sample when compared to static spent (71.71 mg GAE/ g sample) while its mycelia had 59.21 mg GAE/g sample. Mycochemical screening revealed that both mycelial mats contain varying amount of mycochemicals such as flavonoids, tannins, alkaloids and steroids, essential oils, fatty acids, coumarines, phenols, and anthraquinones. Saponins, terpenoids and cardiac glycosides were not detected in both samples.