Association of MHC II DRB3 Polymorphism with subclinical mastitis in lactating goat (Capra hircus) [manuscript]
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Date
2019
Authors
Irvin B. Tabin
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Abstract
The diversity of MHCII DRB3 is essential because of its function in adaptive immunity and its association with infectious diseases resistance or tolerance. This study was conducted to assess the association of polymorphism of MHCII DRB3 genes of goats in selected goat farm in Region III. A total of 45 milk samples were randomly and manually collected. The collected samples were subjected to California Mastitis Test for confirmation of subclinical mastitis. DNA was extracted from milk samples using modified Promega protocol then MHCII DRB3 gene was amplified through PCR. The amplified MHC II DRB3 PCR products were subjected to endonuclease digestion. Sca-1, a restriction enzyme that can cut fragments were analyzed using Sequence Manipulation Suite. Fragments of restriction was resolved on 2-3% agarose gel horizontal electrophoresis. UV transilluminator were used to visualized the restriction-digested gene fragments.
Based on the result of California Mastitis Test (CMT), out of 45 samples 25 samples were classified under subclinical mastitis while 14 samples are negative.
In identification of MHC II DRB3 genotype, a total of 6 smaples was observed to produce single cut and a total of 33 samples produced three cuts in endonuclease restriction enzyme, Sca-1. Chi-square analysis showed no significant relation of the identified genotype to the occurrence of subclinical mastitis in goats.