Isolation and characterization of mungbean rhizobia grown from selected soils in Western Pangasinan [manuscript]
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Date
2017
Authors
Alfred Konrad G. Aquino
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Abstract
This study was conducted to isolate and characterize rhizobia grown from selected soils of western Pangasinan, Mungbean rhizobium was evaluated to understand their ecology and relatedness from one area to another. Twenty four isolates were tested for their morphological characteristics in YMA with Congo red and YMA with BTB. Rhizobium isolates were characterized as faintly pink to white, circular in shape with colony size of 0.5 mm to 3 mm diameter, opaque and translucent. Sixteen out of 24 isolates were fast-growing and acid-producing bacteria classified as Rhizobium while five isolates were slow-growing and alkali-producing bacteria classified as Bradyrhizobium. All rhizobial isolates are Gram negative short rods. Out of 24 isolates, 3 were amplified under the gel documentation map. LII from Lingayen 1, BAY 1 from Bayambang 1 and MABI from Mabini 1. LII is Bradyrhizobium while BAY 1 and MABI are both Rhizobium. Correlation analysis between pH and nodulation of mungbean showed that there is a substantial relationship while EC, OM, soil texture and nodulation of mungbean do not have relationship. Sixty-seven percent of the soils grown to mungbean in the 10 municipalities of western Pangasinan are dominated by Rhizobium bacteria. Mungbean can be nodulated both by Rhizobiun and Bradyrhizobium.