Isolation and analysis of lignin-degrading bacteria from ruminant dung [manuscript]
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Date
2017
Authors
Alexandra R. Agpaoa
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Abstract
Lignin is considered as the most abundant aromatic polymer in nature. It is synthesized by plants such as rice straw which is considered as a plant biomass. Chemical treatments have been used in degration of lignin but this can cause threat to the environment. An isolation and analysis of environment friendly bacteria for lignin degration becomes an essential. Twenty-six bacteria strains have been isolated from cow dung in San Jose City and Science City of Muñoz, Nueva Ecija but only thermoresistant and thermophilic bacteria were selected for further characterization and identification. These isolates were identified as bacillus licheniformis and Proteus sp. and were found to both possess ligninolytic enzyme and can utilize glucose. B. licheniformis was shown its capability in starch hydrolysis, kraft lignin and rice straw degradation but cannot produce bioethanol from rice straw. Also, these potential bacterial strains exhibit plant growth promoting ability which includes phosphorus solubilization and siderophore production but only Proteus sp. can produce indole acetic acid. Furthermore, lignin content of different high yielding varieties of rice was measured through tissue staining and wet chemistry analysis. Total lignin content in both methods were somehow correlated since two of the varieties varied in both methods.