Item: Agro-Morphological characterization and marker assisted detection of gene (HaFAD2-1) in CLSU sunflower gemplasm [manuscript]
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Date
2019
Authors
Christine Joy S. Manalili
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Abstract
Sunflower is one of the most important oil crops which the essential fatty acids of its oil is considered to be important in human diet. There are various sunflower accessions that neccessitate for morphological and oil research. This study aims to evaluate the agro-morphological characteristics of collected sunflower using the guidelines developed by the International Union for the Protection of New Varieties of Plants in order to assess the distinctness, uniformity, and stability among accessions, estimate the morphological diversity using the Shannon & Weaver diversity index and detect the HaFAD2-1 gene in sunflower germplasm. Morphological characteristics clustered the germplasm through agglomerative clustering method. The diversity index (H) range from low to moderate. Out of 20 CLSU sunflower germplasm, 18 accessions had oil extracted. Whereas two accessions namely CL-SF2 and CL-SF20 obtained the highest amount of extracted oil from 50 g of seeds. Sunflower oil content was indirectly correlated and not significantly affected by the physical characteristics of seed, however significant relationship was observed on pollen diameter. detection of HaFAD2-1 gene using gene-specific primers revealed to be present on five out of 20 CLSU sunflower germplasm. The presence of gene can be associated to the amount of oil extracted in two accessions which obtained the highest amount of extracted oil. Thus, HaFAD2-1 gene can be used in the determination of oil type sunflower seed, which can be forwarded to sunflower seed oil production and for breeding purposes.